Thermo Fisher Scientific HELIOS Monoclonal Antibody (22F6), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific HELIOS Monoclonal Antibody (22F6), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (0.00375 µg)/test
• Publications: View 13 publications

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395 / 563 / 737
• Brilliant Violet 421 / 480 / 786
• eFluor 450
• FITC
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• Request custom conjugation

Product Specific Information

This 22F6 monoclonal antibody reacts with human, mouse, and canine Helios. Helios is a zinc-finger transcription factor related to Ikaros, expressed mainly in regulatory T cells but not in mature B cells, dendritic cells, or myeloid cells. It can form homo- and heterodimers with other Ikaros family members to regulate transcription. Some isoforms act as dominant-negative variants and are upregulated in lymphoid malignancies such as T-acute lymphoblastic leukemia.

22F6 has been reported to cross-react with rat Helios.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested using mouse lymph node cells with Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00).

Recommended staining protocol: BestProtocols® - Staining Intracellular Antigens for Flow Cytometry.

Usage: 5 µL (0.00375 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited by a 488 nm blue laser.
Recommended filter: 710/50 bandpass (alternative: 695/40 bandpass).

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Helios is a member of the Ikaros family of zinc-finger proteins (Ikaros, Aiolos, Helios), which are hematopoietic-specific transcription factors regulating lymphocyte development. Helios forms homo- or heterodimers with other family members and functions primarily in early hematopoietic development. Multiple transcript variants exist, though not all have confirmed biological validity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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