Thermo Fisher Scientific F1A alpha Polyclonal Antibody
Rabbit polyclonal antibody targeting human F1A alpha protein. Validated for WB and IHC applications. Unconjugated, liquid form with 0.5 mg/mL concentration. Suitable for apoptosis-related research using mouse liver lysate as positive control.
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Thermo Fisher Scientific F1A alpha Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide amino acids 609–622 of Human F1A alpha. This sequence is identical to the corresponding sequence of mouse. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2102640 |
Product Specific Information
- Recommended positive control: Mouse liver tissue lysate
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was identified and designated F1Aalpha (also known as FEM1-b). F1Aalpha/FEM1-b is the homologue of C. elegans sex-determining protein FEM-1.
FEM-1/F1Aalpha is cleaved by CED-3 and caspase and associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis, making it a novel member of the death receptor-associated proteins mediating apoptosis. F1Aalpha is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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