Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), FITC, eBioscience
CD47 단백질을 인식하는 miap301 클론의 형광 표지 단일클론 항체로, 마우스 시료에서 Flow Cytometry 분석에 최적화됨. FITC 형광(Ex/Em 488/520 nm)을 사용하며 Rat IgG2a, kappa 아이소타입. 고순도 친화 크로마토그래피 정제, 4°C 암소 보관.
- 카탈로그번호
- 11-0471-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), FITC, eBioscience
Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | miap301 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_837105 |
Additional Formats
- Unconjugated
- APC
- Functional Grade
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The monoclonal antibody miap301 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein with a small cytoplasmic domain involved in adhesion through interactions with SIRP (signal regulator protein) and non-covalently associated with β3 integrins (CD51/CD61, CD41/CD61).
It acts as a receptor for thrombospondin and mediates T cell activation or apoptosis under certain conditions.
Expression is found in most hematopoietic cells (T cells, B cells, monocytes, platelets, erythrocytes) and in non-hematopoietic cells.
No cross-reactivity is observed with human CD47.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test; titration recommended for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with integrins and regulating their functions.
It modulates signaling pathways, including heterotrimeric G protein activation, and increases intracellular calcium upon adhesion to extracellular matrix.
CD47 serves as a receptor for the C-terminal domain of thrombospondin, contributing to membrane transport and signal transduction.
CD47 is broadly distributed across tissues, reduced in Rh erythrocytes, and acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells.
Additionally, it influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, being abundant in synapse-rich regions of brain and retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.