Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), FITC, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), FITC, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• APC
• Functional Grade
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody miap301 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein with a small cytoplasmic domain involved in adhesion through interactions with SIRP (signal regulator protein) and non-covalently associated with β3 integrins (CD51/CD61, CD41/CD61).
It acts as a receptor for thrombospondin and mediates T cell activation or apoptosis under certain conditions.

Expression is found in most hematopoietic cells (T cells, B cells, monocytes, platelets, erythrocytes) and in non-hematopoietic cells.
No cross-reactivity is observed with human CD47.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test; titration recommended for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with integrins and regulating their functions.
It modulates signaling pathways, including heterotrimeric G protein activation, and increases intracellular calcium upon adhesion to extracellular matrix.
CD47 serves as a receptor for the C-terminal domain of thrombospondin, contributing to membrane transport and signal transduction.

CD47 is broadly distributed across tissues, reduced in Rh erythrocytes, and acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells.
Additionally, it influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, being abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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