Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), FITC, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody B6H12 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins (CD51/CD61 and CD41/CD61).
It mediates bidirectional signaling to modify neural synaptic activity and regulate macrophage phagocytosis. CD47 acts as a receptor for thrombospondin, influencing T cell activation or apoptosis depending on thrombospondin levels.

Stimulation of CD47 by monoclonal antibody induces CD4+CD25- suppressive activity and increases Foxp3 expression.
Expression is found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and also in non-hematopoietic cells.
This antibody has neutralizing activity.

Applications Reported: Flow cytometric analysis and IHC staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells.
Recommended use: 5 µL (1 µg) per test (100 µL final volume).
Excitation: 488 nm, Emission: 520 nm, Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with integrins and regulates their functions.
Engagement of CD47 modulates signaling pathways such as activation of heterotrimeric G proteins and increases intracellular calcium upon adhesion to extracellular matrix.
CD47 serves as a receptor for the C-terminal domain of thrombospondin and plays roles in membrane transport and signal transduction.

CD47 expression is broad across tissues, reduced on Rh erythrocytes, and acts as a ligand for CD172a (SIRPα) on macrophages, preventing phagocytosis of CD47-positive cells.
The CD47–CD172a system affects cell migration, B cell adhesion, T cell activation, chondrocyte mechanical response, and neuronal development, especially in synapse-rich regions of the brain and retina.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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