Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody 2D3 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein with a small alternatively spliced cytoplasmic domain. It is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins (CD51/CD61 and CD41/CD61). These interactions mediate bi-directional signaling, modifying neural synaptic activity and regulating macrophage phagocytic activity.

CD47 serves as a receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis in the presence of high thrombospondin levels. Expression is found in most hematopoietic cells, including T and B cells, monocytes, platelets, and erythrocytes, as well as in non-hematopoietic cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells.
Use at 5 µL (1 µg) per test (in 100 µL final volume). Cell count may vary between 10⁵–10⁸ cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with integrins and regulates their functions. Engagement of CD47 by ligands or counter receptors modulates various signaling pathways, including activation of heterotrimeric G proteins.

Functionally, CD47 is involved in increasing intracellular calcium concentration upon cell adhesion to the extracellular matrix. It also acts as a receptor for the C-terminal cell-binding domain of thrombospondin and may play roles in membrane transport and signal transduction.

CD47 is broadly distributed across tissues and reduced on Rh erythrocytes. It acts as a ligand for CD172a (SIRPα), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. The CD47–CD172a system influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, being abundant in synapse-rich brain and retinal regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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