Thermo Fisher Scientific CDK4 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Antiserum specifically reacts with a 34 kDa CDK4 (p34) protein from human, rat, and mouse tissue. No reaction was observed against other related cyclin-dependent kinases. Cross-reactivity with CDK4 (p34) from other species has not been determined.

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of the cell cycle. CDK4 forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein, leading to active transcriptional repression of the E2F complex. CDC37 and HSP90 preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on the Rb protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.