Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Yellow 730, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Usage: 5 µL (0.6 µg)/test

Product Specifications

Available Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• Super Bright 436
• Custom conjugation available

Product Specific Information

Description:
Monoclonal antibody 2D3 recognizes CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein involved in cell adhesion via interaction with SIRP and integrins (CD51/CD61, CD41/CD61). It mediates bi-directional signaling affecting neural synaptic activity and macrophage phagocytosis. CD47 serves as a receptor for thrombospondin and is expressed on most hematopoietic cells (T/B cells, monocytes, platelets, erythrocytes) and various non-hematopoietic cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.6 µg) per test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of blocking buffer per sample, regardless of the number of NovaFluor conjugates.
• For single-color controls: 5 µL buffer per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Spectral Properties:

• Excitation: 552 nm
• Emission: 718 nm
• Laser: 561 nm (Yellow)

Target Information

CD47 (Integrin-Associated Protein, IAP) is a transmembrane glycoprotein interacting with integrins and modulating signaling pathways, including G protein activation and intracellular calcium increase upon adhesion. It acts as a receptor for thrombospondin and ligand for CD172a (SIRPα), inhibiting phagocytosis of CD47-positive cells. CD47 influences cell migration, B/T cell adhesion, chondrocyte mechanotransduction, and neuronal development, with high expression in synapse-rich brain and retina regions.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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