Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Red 725

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 690
• NovaFluor Red 710
• NovaFluor Yellow 570 / 660 / 730 / 755
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody 2D3 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins CD51/CD61 and CD41/CD61. It mediates bi-directional signaling, regulates macrophage phagocytosis, and acts as a receptor for thrombospondin.

Expression is found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using human peripheral blood cells. Use 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), optimized for NovaFluor staining. Add 5 µL per sample prior to combining with cells. For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures with reduced spillover and improved spectral performance.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD47 (integrin-associated protein, IAP) is a transmembrane glycoprotein interacting with integrins and regulating their functions. It modulates signaling pathways, affects calcium mobilization upon adhesion, and serves as a receptor for thrombospondin. CD47 is widely expressed and reduced on Rh erythrocytes. It acts as a ligand for CD172a (SIRPα), preventing macrophage phagocytosis. CD47-CD172a interaction influences migration, adhesion, activation, chondrocyte mechanotransduction, and neuronal development.

For Research Use Only. Not for use in diagnostic procedures.

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