Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), eBioscience
IRF4 단백질을 인식하는 Rat IgG1 단클론 항체로, 인간 및 마우스 시료에 반응합니다. Western blot에 검증되었으며, B세포·T세포·대식세포 기능 연구에 적합합니다. 액상 형태로 제공되며, 0.5 mg/mL 농도 및 PBS 버퍼에 보존됩니다.
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Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 19 publications |
| ELISA | - | View 1 publication |
| ChIP assay | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865481 |
Available Conjugated Formats
- Alexa Fluor 700
- Brilliant Violet 480
- Brilliant Violet 650
- eFluor 450
- eFluor 660
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.
- In B cells, IRF4 is highly expressed in mature plasma cells and regulates differentiation, interacting with PU.1 and controlling transcription of B cell–specific genes such as Prdm1 (Blimp1).
- In T cells, IRF4 contributes to Treg, Th2, Th9, and Th17 cell development and function. It is upregulated upon activation and induces Blimp1 and IL-10 expression.
- IRF4 also plays a role in macrophage polarization and is upregulated in several hematologic malignancies.
Applications Reported: Western blotting.
Applications Tested: Western blot on reduced splenocyte lysate cultured with plate-bound Anti-Mouse CD3 and soluble Anti-Mouse CD28. Recommended ≤ 5 µg/mL; titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The IRF (interferon regulatory factor) family of transcription factors share a tryptophan pentad repeat DNA-binding domain. They regulate interferon responses and interferon-inducible genes.
IRF4 is lymphocyte-specific and negatively regulates TLR signaling, essential for innate and adaptive immunity.
A chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH may cause multiple myeloma.
Alternatively spliced transcript variants have been identified.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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