Thermo Fisher Scientific IL-25 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930843

Product Specific Information

AA Sequence of recombinant protein: MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17E. Anti-Human IL-17E-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human IL-17E by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17E.

Western Blot: To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL.andnbsp; When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17E is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P89BT-1MG will be provided as 2 x 500 µg

Target Information

Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.