Thermo Fisher Scientific IL-25 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17E. Anti-Human IL-17E-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IL-17E by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-17E.

Western Blot:
To detect Human IL-17E by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-17E is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P89BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 25 (IL-25), also known as IL-17E, is a member of the IL-17 family and functions as a proinflammatory cytokine produced by Th17 effector cells. It plays a role in initiating or maintaining autoimmune processes in diseases such as rheumatoid arthritis. IL-25 induces activation of NF-κB and stimulates production of the proinflammatory chemokine IL-8. It is itself activated through IL-23, IL-5, and TGF-β.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.