Thermo Fisher Scientific IL-17AF Monoclonal Antibody (B8KN8R), PE, eBioscience
마우스 IL-17AF 헤테로다이머를 특이적으로 인식하는 단클론 항체로, IL-17A/F 호모다이머에는 반응하지 않음. Flow Cytometry용으로 검증됨. PE 형광체 결합, 488–561 nm 여기 및 578 nm 방출. 0.25 µg/test로 사용 권장, 4°C 암저장.
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Thermo Fisher Scientific IL-17AF Monoclonal Antibody (B8KN8R), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested dilution: 0.25 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B8KN8R |
| Immunogen | Recombinant IL-17AF |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572697 |
Product Specific Information
The B8KN8R monoclonal antibody reacts with mouse interleukin (IL)-17AF heterodimer. It specifically binds IL-17AF without cross-reactivity to IL-17A or IL-17F homodimers.
IL-17A and IL-17F are cytokines secreted by Th17 cells, γδ T cells, and innate lymphoid cells. IL-17AF heterodimer signals through the IL-17RA/IL-17RC receptor complex, playing a role in mucosal immune responses and is associated with inflammatory diseases such as IBD, asthma, atopic dermatitis, and rheumatoid arthritis.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Tested on Th17-polarized mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
- Recommended usage ≤ 0.25 µg per test (100 µL final volume).
- Cell number range: 10⁵–10⁸ cells/test.
- Optimize antibody titration for best performance.
Fluorescence Information:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-17A and IL-17F are homodimeric cytokines secreted by Th17 and γδ T cells. They can also form the heterodimer IL-17AF, signaling through IL-17RA/IL-17RC receptors to mediate mucosal immune responses. IL-17AF is implicated in inflammatory diseases including IBD, asthma, atopic dermatitis, and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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