Thermo Fisher Scientific IL-28A Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-28A Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
PVARLHGALP DARGCHIAQF KSLSPQELQA FKRAKDALEE SLLLKDCRCH SRLFPRTWDL RQLQVRERPM ALEAELALTL KVLEATADTD PALVDVLDQP LHTLHHILSQ FRACIQPQPT AGPRTRGRLH HWLYRLQEAP KKESPGCLEA SVTFNLFRLL TRDLNCVASG DLCV

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-lambda2. Anti-Human IFN-lambda2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IFN-lambda2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IFN-lambda2 (500-P247) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IFN-lambda2.

Western Blot:
To detect Human IFN-lambda2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IFN-lambda2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P247BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), also known as type III Interferons.
The IFN lambdas signal through a heterodimeric receptor composed of IL-10R2 and IFN lambda R1 (IL-28R alpha). Signaling occurs via the Jak/STAT pathway, similar to type I IFN (IFN alpha/beta), and activates many of the same genes despite low sequence homology.
Both IFN families display antiviral activity through induction of antiviral proteins and upregulation of MHC class I expression. They also exhibit antiproliferative and antitumor effects, suggesting potential use as alternatives to IFN alpha therapies.
Unlike type I IFN, response to IFN lambda is limited to dendritic and some tumor cells due to restricted expression of IFN lambda R1. IFN lambda stimulation can drive dendritic cells toward CD4+CD25+FoxP3+ regulatory T cell production, indicating a possible immunoregulatory role.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.