Thermo Fisher Scientific Artemin Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Artemin. Anti-Human Artemin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hArtemin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Artemin (500-P245) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hArtemin.

Western Blot:
To detect hArtemin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hArtemin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P245BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a member of the glial cell line-derived neurotrophic factor (GDNF) family of ligands within the TGF-beta superfamily of signaling molecules. GDNFs possess neurotrophic properties and have potential applications in gene therapy for neurodegenerative diseases. Artemin supports the survival of several peripheral neuron populations and dopaminergic CNS neurons. It acts as a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.