Thermo Fisher Scientific HSP90 Monoclonal Antibody (D7A), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Rabbit, Rat, Bovine, Chicken, Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

D7A

Immunogen

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Conjugate

FITC FITC FITC

View additional formats

• APC
• PerCP
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931612

Product Specific Information

2 µg/mL was sufficient for detection of HSP90 alpha in 20 µg of heat shocked HeLa cell lysate as well as in 100 ng of human HSP90 alpha protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Recognizes 90kDa.approximately Can isolate complexes of HSP90, Src kinase and cec37.

Target Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.