Thermo Fisher Scientific ASGR1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human ASGR1 for WB, IHC, ELISA, and IP applications. Recombinant human ASGPR1 immunogen (Gln62–Leu291). Preservative-free, liquid form, 1 mg/mL concentration. Suitable for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human ASGPR1/ASGR1 protein (Gln62–Leu291) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787681 |
Product Specific Information
- Preservative-free formulation.
- Recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
- Specificity: Human ASGPR1/ASGR1.
Target Information
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42 kDa) that mediates binding, internalization, and degradation of extracellular glycoproteins with exposed terminal galactose residues. It is expressed on hepatocyte surfaces in a polar manner—present on sinusoidal and lateral plasma membranes but absent on bile canalicular membranes.
In mammals, hepatic ASGPR mediates endocytosis and degradation of serum proteins lacking terminal sialic residues. The rat ASGPR consists of three subunits: RHL-1, RHL-2, and RHL-3. The monoclonal antibody 8D7 recognizes a subunit-specific epitope of RHL-1 of rat ASGPR and is cross-reactive with human ASGPR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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