Thermo Fisher Scientific Desmosome/Hemidesmosome Recombinant Human Monoclonal Antibody (F12)
인간 유래 단클론 항체(F12)로 데스모좀 및 헤미데스모좀 단백질을 인식. 면역현미경(IM)에 적합하며 HEK293 세포에서 발현된 재조합 항체. 액상 형태, 1 mg/mL 농도, ProClin 300 포함 PBS 저장액. 조직 구조 연구용.
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Applications
- Immunomicroscopy (IM)
Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Human / IgM, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | F12 |
| Immunogen | F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a molecular weight of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate).
By immunoelectron microscopy (IEM), it was determined that F12 binds to the intracellular part of the desmosome.
Target Information
Desmosomes and hemidesmosomes are two types of intercellular junctions that play important roles in maintaining the structural integrity of tissues subjected to mechanical stress or shear forces.
- Desmosomes connect neighboring cells using cadherins and intermediate filaments.
- Hemidesmosomes anchor epithelial cells to the underlying extracellular matrix using integrins.
Both junctions are commonly found in tissues such as skin, heart, and muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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