Thermo Fisher Scientific SSEA1 Recombinant Mouse Monoclonal Antibody (BRA-4F1)
Recombinant mouse monoclonal antibody (clone BRA-4F1) specific for CD15 (SSEA-1). Suitable for IHC, ICC/IF, Flow Cytometry, and IP. Expressed in HEK293 cells and purified by affinity chromatography. Supplied as liquid, 1 mg/mL in PBS with 0.02% ProClin...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BRA-4F1 |
| Immunogen | This antibody was raised by immunizing mice with human CD15. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
Specificity: This antibody is specific for CD15 and shows cross-reactivity with the CD15 antigen from other species.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types, including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells. Variants of Lewis X include sialyl-Lewis X and sulphated Lewis X. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation based on calcium-dependent Le(x)-Le(x) interactions, which are involved in processes such as embryo compaction and teratocarcinoma cell aggregation. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression is widely used to confirm the diagnosis of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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