Thermo Fisher Scientific Phospho-c-Kit (Ser746) Polyclonal Antibody
Phospho-c-Kit (Ser746) 폴리클로날 항체로, 인산화된 c-Kit 단백질의 Ser746 부위를 특이적으로 인식합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 사람, 생쥐, 랫트 시료에 반응합니다. 액상 형태로 -20°C에서 보관합니다.
- 카탈로그번호
- PA5105894
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-c-Kit (Ser746) Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human KIT (Accession P10721), corresponding to amino acid residues around phosphorylated Ser746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817293 |
Product Specific Information
This antibody detects endogenous levels of c-Kit only when phosphorylated at Ser746.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). It regulates the growth and activation of various hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT receptor TK by the D816V mutation has become a therapeutic target for neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Upon binding of its ligand SCF, c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues, including Tyr703. This enables docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-Kit can also activate PI3K, PLC-γ, and JAK/STAT pathways. c-Kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.