Thermo Fisher Scientific FH Monoclonal Antibody (2C5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

2C5

Immunogen

FH (AAH03108, 33 a.a. approximately 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Fumarase,uniProtId:P07954-1,ncbiNodeId:9606,antigenRange:33-510,antigenLength:510,antigenImageFileName:H00002271-M03_Fumarase_P07954-1_House_mouse.svg,antigenImageFileNamePDP:H00002271-M03_Fumarase_P07954-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: VPSFWPPNAA RMASQNSFRI EYDTFGELKV PNDKYYGAQT VRSTMNFKIG GVTERMPTPV IKAFGILKRA AAEVNQDYGL DPKIANAIMK AADEVAEGKL NDHFPLVVWQ TGSGTQTNMN VNEVISNRAI EMLGGELGSK IPVHPNDHVN KSQSSNDTFP TAMHIAAAIE VHEVLLPGLQ KLHDALDAKS KEFAQIIKIG RTHTQDAVPL TLGQEFSGYV QQVKYAMTRI KAAMPRIYEL AAGGTAVGTG LNTRIGFAEK VAAKVAALTG LPFVTAPNKF EALAAHDALV ELSGAMNTTA CSLMKIANDI RFLGSGPRSG LGELILPENE PGSSIMPGKV NPTQCEAMTM VAAQVMGNHV AVTVGGSNGH FELNVFKPMM IKNVLHSARL LGDASVSFTE NCVVGIQANT ERINKLMNES LMLVTALNPH IGYDKAAKIA KTAHKNGSTL KETAIELGYL TAEQFDEWVK PKDMLGPK

Target Information

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.