Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (3F7), FITC
7-Ketocholesterol 특이적 단클론 항체로 FITC 형광 표지된 제품. WB, ICC/IF, ELISA 등 다양한 응용 가능. 단백질 G로 정제된 고순도 항체. 4°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (3F7), FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F7 |
| Immunogen | Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 581 mM DMSO, 123 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932252 |
Additional Formats
- Unconjugated (MA5-27561)
- APC (MA5-45797)
- PE (MA5-45800)
- PerCP (MA5-45799)
- Request custom conjugation available
Product Specific Information
A 1:1,000 dilution of MA5-45798 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 7-ketocholesterol modified proteins.
Target Information
7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies.
It can inhibit cholesterol 7α-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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