Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (7E1), PE
7-Ketocholesterol 단백질을 특이적으로 검출하는 PE 형광 표지 단일클론 항체. Western blot, ICC/IF, ELISA에 적합하며, 마우스 IgG2b 아이소타입. 565/576 nm 형광 특성으로 고감도 분석 가능.
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Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (7E1), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E1 |
| Immunogen | Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932250 |
Available Formats
- Unconjugated (MA5-27571)
- APC (MA5-45793)
- FITC (MA5-45794)
- PerCP (MA5-45795)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45796 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 7-ketocholesterol modified proteins.
Target Information
7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies.
It can inhibit cholesterol 7α-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibit HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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