Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (7E1), FITC
7-Ketocholesterol에 특이적인 단클론 항체로, FITC 형광 표지. Western blot, ICC/IF, ELISA에 적합. 단백질 산화 연구 및 죽상동맥경화 관련 연구에 활용 가능. 4°C 암소 보관, 보존제 없음.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E1 |
| Immunogen | Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932248 |
Additional Formats
- Unconjugated (MA5-27571)
- APC (MA5-45793)
- PE (MA5-45796)
- PerCP (MA5-45795)
- Custom conjugation available
Product Specific Information
A 1:1,000 dilution of MA5-45794 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 7-ketocholesterol modified proteins.
Target Information
7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies.
It can inhibit cholesterol 7α-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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