Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience™

Applications and Tested Dilution

• Applications Tested: Intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells
• Recommended Dilution: ≤ 0.5 µg/test
  • A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
  • Antibody titration recommended for optimal performance

Publications

• View 34 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 650
• eFluor 450
• FITC
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists in a 26 kDa membrane-bound form.
This MAb11 antibody is a neutralizing antibody suitable for intracellular staining and flow cytometric analysis.

• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF alpha regulates immune responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as multimers (dimers, trimers, pentamers) and shows a single 17 kDa band under non-reducing SDS-PAGE.
Knockout studies in mice indicate TNF alpha’s neuroprotective function and tumor necrosis activity.
It is implicated in autoimmune diseases, insulin resistance, and cancer, and plays roles in immune responses to bacterial, viral, parasitic, and fungal infections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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