Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), PE, eBioscience
TNF alpha 단클론 항체(TN3-19.12), PE 형은 마우스 및 랫트 TNFα에 반응하며 중화 기능을 가집니다. Flow cytometry 및 ELISA에 적합하며, 고순도(>90%)로 정제된 항체입니다. 565/576 nm 형광 특성을 가지며 4°C 암소 보관이 필요합니다.
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Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), PE, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TN3-19.12 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11149178 |
Additional Formats
Product Specific Information
The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit TNF alpha, but not with human TNF alpha. It is a neutralizing antibody.
Mouse TNF alpha (17 kDa) is produced by macrophages, monocytes, neutrophils, CD4+ T cells, and NK cells. A 26 kDa membrane-bound form also exists. TNF alpha plays an important role in immune regulation and cytolytic activity. Dimers and trimers have been observed.
Applications Reported:
- Intracellular staining for flow cytometric analysis
- Cytokine neutralization
- ELISA
Fluorochrome-conjugated TN3-19.12 is recommended for intracellular staining and flow cytometry. Functional Grade purified TN3-19.12 (Product #13-4813-85) is available. Other secondaries may yield suboptimal staining.
Titrate carefully for optimal assay performance.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90%, determined by SDS-PAGE.
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10%, determined by HPLC.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine of the TNF superfamily. Secreted mainly by macrophages, it binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
It regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as multimers and shows a 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout studies suggest its neuroprotective function. TNF alpha induces tumor necrosis, contributes to immune responses against infections, and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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