Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Biotin, eBioscience
인간 CD86(B7-2)을 인식하는 IT2.2 클론의 비오틴 결합 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. 항원제시세포의 활성화 및 T세포 공동자극 연구에 적합하며, 고순도 친화성 크로마토그래피 정제 및 안정적인 액상 형태로 제공됩니다.
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Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 21 publications |
| Neutralization (Neu) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IT2.2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466529 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor 700
- APC
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 711
- Brilliant Violet 786
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 690
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated through various stimuli including BCR complex, CD40, and cytokine receptors.
CD86 interacts with CD28 and CD152 (CTLA-4) on T cells, playing a critical role in T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production.
The IT2.2 antibody is validated for flow cytometric analysis of normal human peripheral blood cells and can be used at ≤0.5 µg per test (100 µL final volume). Optimal titration is recommended for best results.
Target Information
CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Insufficient co-stimulation via CD86/CD80 can lead to immune tolerance.
CD86 is a type I membrane protein in the immunoglobulin superfamily, expressed by antigen-presenting cells. Binding of CD86 with CD28 provides a stimulatory signal, while interaction with CTLA-4 inhibits T-cell activation.
Alternative splicing results in multiple isoforms. CD86 dysfunction has been associated with gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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