Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated through stimuli such as BCR complex, CD40, and cytokine receptors. It is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells.

CD86, along with CD80 (B7-1), is a counter-receptor for T-cell surface molecules CD28 and CD152 (CTLA-4). This interaction is essential for T-B cell crosstalk, T-cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation supports its major role during the primary phase of an immune response.

Applications Reported: The GL1 antibody is reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended ≤0.25 µg per test in a 100 µL staining volume (cell number 10⁵–10⁸/test). Antibody titration is recommended for optimal performance.

Target Information

CD86 is one of two ligands (the other being CD80) for CTLA4 and CD28. It functions as a costimulatory molecule in eliciting T-cell help during antigen presentation. Insufficient co-stimulation involving CD86/CD80 can induce tolerance.

CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells. It binds to CD28 and CTLA-4 on T cells, providing positive or negative regulation of T-cell activation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.