Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 645, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 645, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV (395–805 series)
• Brilliant Violet (421–786 series)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue and Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 702, 780
• Request custom conjugation available

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that associates with CD18 to form Mac-1 (αMβ2 integrin). It binds CD54 (ICAM-1), C3bi, and fibrinogen, and is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells.
This antibody cross-reacts with human CD11b and can detect the antigen on human monocytes, granulocytes, and NK cell subsets.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.125 µg/test (100 µL final volume). Optimize titration for best performance.

Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Recommended detection filter: 660/20 bandpass.

Usage Notes:

• Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
• Protect vial and samples from light (photo-induced oxidation sensitivity).
• Samples can be fixed using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) and stored up to 3 days at 4°C in the dark.
• Excitation: 405 nm; Emission: 645 nm; Laser: Violet.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
CD11b/CD18 serves as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating key cell-cell and cell-matrix interactions.
Expression varies by tissue and cell type and is commonly used as a microglial marker. Dysregulation is linked to systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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