Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 645, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 645, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication

Product Specifications

Additional Formats Available

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue / Red / Yellow series (various wavelengths)
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Custom conjugation available upon request

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (FcγRIII), which exists as two isoforms: FcγRIIIA and FcγRIIIB.

• Expression: FcγRIIIA on monocytes, macrophages, NK cells; FcγRIIIB on neutrophils.
• Function: Mediates ADCC, phagocytosis, cytokine release, and antigen presentation.
• Epitope: 3G8 recognizes the same epitope as CB16 but distinct from B73.1.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm.
Use a 660/20 bandpass filter. Ensure the instrument detects this fluorochrome.

Staining Recommendations:
When using multiple Super Bright conjugates, employ Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Protect vial and samples from light due to photo-induced oxidation sensitivity.

Fixation:
Samples may be fixed using IC Fixation Buffer (Product # 00-8222) or 1-Step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, facilitating immune complex clearance and cellular cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expression: NK cells (transmembrane form), neutrophils (PI-anchored form via FCGR3B)
• Clinical relevance: Mutations linked to immunodeficiency 20, herpes zoster, lupus, and neonatal neutropenia.
  Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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