Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 645, eBioscience
인간 및 비인간 영장류 CD16(FcγRIII) 검출용 3G8 단클론 항체로, Super Bright 645 형광체 결합. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기, 645 nm에서 방출. 빛에 민감하며, Super Bright 완전 염색 버퍼 사용 권장.
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Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 645, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 645 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G8 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717084 |
Additional Formats Available
- APC-eFluor 780
- Functional Grade
- NovaFluor Blue / Red / Yellow series (various wavelengths)
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Custom conjugation available upon request
Product Specific Information
This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (FcγRIII), which exists as two isoforms: FcγRIIIA and FcγRIIIB.
- Expression: FcγRIIIA on monocytes, macrophages, NK cells; FcγRIIIB on neutrophils.
- Function: Mediates ADCC, phagocytosis, cytokine release, and antigen presentation.
- Epitope: 3G8 recognizes the same epitope as CB16 but distinct from B73.1.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorophore Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm.
Use a 660/20 bandpass filter. Ensure the instrument detects this fluorochrome.
Staining Recommendations:
When using multiple Super Bright conjugates, employ Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Protect vial and samples from light due to photo-induced oxidation sensitivity.
Fixation:
Samples may be fixed using IC Fixation Buffer (Product # 00-8222) or 1-Step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD16 encodes a receptor recognizing the Fc portion of IgG, facilitating immune complex clearance and cellular cytotoxicity.
- Gene: FCGR3A (chromosome 1)
- Expression: NK cells (transmembrane form), neutrophils (PI-anchored form via FCGR3B)
- Clinical relevance: Mutations linked to immunodeficiency 20, herpes zoster, lupus, and neonatal neutropenia.
Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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