Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Super Bright 645, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 16 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661, 737, 805
• Brilliant Violet 480
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, 725
• NovaFluor Yellow 660, 730
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 702, 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes:

• CD16A (FcγRIIIA): Found in macrophages and NK cells (transmembrane form)
• CD16B (FcγRIIIB): Found in neutrophils (GPI-anchored form)

Polymorphisms NA-1 and NA-2 exist for CD16B, with NA-2 homozygotes showing lower phagocytic capacity.
CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for human peripheral blood cells at 5 µL (0.125 µg)/test (in 100 µL total volume, 10⁵–10⁸ cells/test). Optimal titration is recommended.

Fluorochrome Information:

• Super Bright 645 is a tandem dye excited by 405 nm laser, emits at 645 nm
• Recommended filter: 660/20 bandpass
• Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes
• Light sensitive – protect from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD16 (FcγRIII) encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance, antibody-dependent cytotoxicity, and viral infection enhancement.

• Gene: FCGR3A (chromosome 1), similar to FCGR3B
• Expression:
  • FCGR3A: NK cells (transmembrane form)
  • FCGR3B: Neutrophils (PI-anchored form)
• Mutations linked to Immunodeficiency 20, Herpes Zoster, Systemic Lupus Erythematosus, and Alloimmune Neonatal Neutropenia
• Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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