Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421, 650, 786
• eFluor 450, 506, 660, FITC
• Functional Grade
• NovaFluor Red 700, Yellow 610
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, also known as Gr-1, a 21–25 kDa GPI-linked protein expressed in the myeloid lineage. Gr-1 is transiently expressed in monocytes during bone marrow development and is a reliable marker for granulocytes and peripheral neutrophils.

eBioscience testing shows that RB6-8C5 also stains cells expressing high levels of Ly6C. For Ly-6G-specific detection, use clone 1A8-Ly6G (Product # 9668).

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells (≤0.25 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Brilliant Violet™ 711 (BV711) emits at 713 nm, suitable for cytometers with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to reduce non-specific polymer interactions when using multiple polymer dye-conjugated antibodies.

Light Sensitivity: Protect vial and samples from light.
Fixation: Compatible with IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-54) for up to 3 days at 4°C in dark.
Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Granulocytes are white blood cells containing cytoplasmic granules and are often referred to as polymorphonuclear leukocytes (PMN) due to their multi-lobed nuclei. Neutrophils are the most abundant granulocytes. These cells are released from bone marrow under regulation by complement proteins.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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