Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 711

Applications

• Flow Cytometry (intracellular staining)

Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 786
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The eBio17B7 antibody reacts specifically with mouse and rat IL-17A, showing no cross-reactivity with IL-17F. IL-17A is a CD4⁺ T cell-derived cytokine involved in inflammatory responses and elevated in autoimmune conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested: Validated using mouse splenocytes with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage ≤ 0.25 µg per test (100 µL sample volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is optimized for cytometers equipped with a 405 nm violet laser. Use Super Bright or Brilliant Stain Buffer to minimize polymer dye interactions.

Handling Notes:

• Light-sensitive; protect from light during storage and staining.
• Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C.
• Not compatible with methanol-based fixation.
• Excitation: 407 nm; Emission: 713 nm; Laser: Violet.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4⁺ T cell-derived cytokine that promotes inflammatory responses and is implicated in autoimmune diseases. It forms a disulfide-linked homodimer (32 kDa, 136 amino acids) and signals through IL-17R/CDw217. IL-17A enhances ICAM-1 expression and stimulates secretion of IL-6, IL-8, G-CSF, and PGE2, supporting hematopoietic progenitor proliferation and neutrophil maturation. It regulates NF-κB and MAPK pathways, increasing IL-6 and COX-2 expression and nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures.

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