Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. Generated using the TE-71 thymic epithelial cell line, CD326 is expressed on most epithelial cells and considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may function as a growth factor receptor. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306), inhibiting activation and inflammation, and plays a key role in tumor biology.

Applications Reported:
This G8.8 antibody is suitable for flow cytometric analysis.

Applications Tested:
Validated in flow cytometric analysis of TE-71 cells. Recommended ≤0.125 µg per test (100 µL final volume). Cell count may vary from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.

Brilliant Violet™ 711 (BV711):
A tandem dye emitting at 713 nm, suitable for cytometers with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
Light-sensitive — protect vial and samples from light.
Not compatible with methanol-based fixation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in dark with minimal brightness loss.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a homotypic, Ca²⁺-independent adhesion molecule and influences cell cycle, proliferation, and metabolism via c-myc, cyclin A, and cyclin E induction. Ep-CAM overexpression correlates with enhanced epithelial proliferation and is highly expressed in carcinomas, serving as a marker for epithelial lineage tumors. It is localized on basolateral surfaces of most simple epithelia and carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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