Thermo Fisher Scientific Phospho-eNOS (Ser1177) Recombinant Rabbit Monoclonal Antibody (PS01-10)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200

Product Specifications

Product Specific Information

• Sequence Similarities: No cross-reactivity with Mouse and Rat
• Tissue Specificity: Platelets, placenta, liver, and kidney
• Positive Control: HAEC serum starved then treated with 100 nM insulin for 1 hour; human placenta tissue
• Subcellular Location: Cell membrane, caveola, cytoplasm, cytoskeleton, Golgi apparatus
• Predicted Band Size: 133 kDa

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that mediates various intercellular signals such as vasorelaxation, neurotransmission, and cytotoxicity. NO production is catalyzed by nitric oxide synthase (NOS), which converts L-arginine to L-citrulline and NO.
Three NOS isoforms have been identified:

• nNOS (type I): neuronal NOS
• iNOS (type II): inducible NOS
• eNOS (type III): endothelial NOS

All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.