Thermo Fisher Scientific Phospho-eNOS (Ser1177) Recombinant Rabbit Monoclonal Antibody (PS01-10)
Phospho-eNOS (Ser1177) 특이적 재조합 단일클론 항체로 인간 시료에 반응합니다. Western blot 및 IHC(P) 검증 완료. 고순도 Protein A 정제, 안정적 액상 형태로 제공. 세포막 및 세포골격 내 eNOS 인산화 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PS01-10 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser1177 of human eNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092987 |
Product Specific Information
- Sequence Similarities: No cross-reactivity with Mouse and Rat
- Tissue Specificity: Platelets, placenta, liver, and kidney
- Positive Control: HAEC serum starved then treated with 100 nM insulin for 1 hour; human placenta tissue
- Subcellular Location: Cell membrane, caveola, cytoplasm, cytoskeleton, Golgi apparatus
- Predicted Band Size: 133 kDa
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that mediates various intercellular signals such as vasorelaxation, neurotransmission, and cytotoxicity. NO production is catalyzed by nitric oxide synthase (NOS), which converts L-arginine to L-citrulline and NO.
Three NOS isoforms have been identified:
- nNOS (type I): neuronal NOS
- iNOS (type II): inducible NOS
- eNOS (type III): endothelial NOS
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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