Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2466)
인간 Ki-67 단백질을 인식하는 Mouse monoclonal 항체로, 세포 증식 지표 분석에 사용됩니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, Protein A/G 정제된 고순도 항체입니다. 연구용으로만 사용 가능합니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2466)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2466 |
| Immunogen | Recombinant fragment of human Ki67 protein (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus. Ki-67 shows cell cycle–dependent localization: perinucleolar expression at G1, nuclear matrix expression at G2, and chromosomal expression during M phase.
Ki-67 is widely used as a proliferation marker because it is absent in G0 cells and increases from G1 through mitosis. Ki-67 antibodies help determine the proliferative fraction of cells in neoplasms, correlating with tumor grade and prognosis. It is also used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.