Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2466)
인간 Ki-67 단백질을 인식하는 마우스 모노클로날 항체로, 세포 증식 마커 연구에 적합. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태, 4°C 보관.
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- 4288-MSM6-P1
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- pk
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2466)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2466 |
| Immunogen | Recombinant fragment of human Ki67 protein (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that enables interaction with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
Because Ki-67 is absent in G0 cells and increases throughout the cell cycle, it serves as a reliable proliferation marker. Ki-67 antibodies are used to determine the cell growth fraction in neoplasms, with prognostic value comparable to the thymidine-labeling index. Low Ki-67 index correlates with histologically low-grade tumors.
It is routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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