Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2465)
인간 Ki-67 단백질을 인식하는 마우스 단클론 항체로 세포 증식 마커 연구에 사용됨. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태, 보존제 미포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2465 |
| Immunogen | Recombinant fragment (aa 2293–2478) of human Ki-67 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Expression is cell cycle dependent—perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
Ki-67 serves as a proliferation marker, absent in G0 cells and increasing steadily through mitosis. It is used to assess cell growth fraction in neoplasms, with prognostic value comparable to thymidine-labelling index.
Low Ki-67 index correlates with histologically low-grade tumors. Commonly used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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