Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Biotin, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV, Violet, NovaFluor, PE series
• FITC, eFluor, Super Bright conjugates
• Functional Grade
• Custom conjugation available upon request

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells. Crosslinking via UCHT1 triggers intracellular signaling leading to activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry of normal human peripheral blood cells (≤ 1 µg/test)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded on chromosome 11. They associate with signal transduction molecules via a tyrosine-based motif. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. Defects in CD3 genes can lead to immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.