Thermo Fisher Scientific Clusterin/Apolipoprotein J (APO-J) Monoclonal Antibody (CLU, 4731)
Thermo Fisher Scientific의 Clusterin/Apolipoprotein J (APO-J) 단일클론 항체(CLU, 4731)는 인간 반응성 마우스 IgG2a 항체로, WB 및 IHC(P) 등 다양한 응용에 적합합니다. 단백질 응집 방지와 세포 보호 관련 연구에 활용되며, 4°C에서 보관됩니다.
- 카탈로그번호
- 1191-MSM11-P1
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CLU, 4731 |
| Immunogen | Recombinant fragment (around aa150–300) of human CLU protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Isoform 1 functions as an extracellular chaperone that prevents aggregation of nonnative proteins and stress-induced aggregation of blood plasma proteins. It inhibits amyloid fibril formation by APP, APOC2, B2M, CALCA, CSN3, SNCA, and aggregation-prone LYZ variants in vitro.
Clusterin does not require ATP and maintains partially unfolded proteins suitable for refolding by other chaperones such as HSPA8/HSC70. It does not refold proteins by itself.
Binding to cell surface receptors triggers internalization and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and complement-mediated cytolysis.
Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, promoting ubiquitination and proteasomal degradation of target proteins.
Clusterin modulates NF-kappa-B transcriptional activity, with nuclear isoforms promoting apoptosis and mitochondrial isoforms inhibiting BAX-dependent cytochrome c release, thereby suppressing apoptosis.
It plays an important role in cell proliferation regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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