Thermo Fisher Scientific Clusterin/Apolipoprotein J (APO-J) Monoclonal Antibody (CLU, 4731)
Clusterin/Apolipoprotein J 단백질을 인식하는 Mouse monoclonal antibody로, WB, IHC(P), Peptide Array에 사용 가능. Human 시료에 반응하며 Protein A/G 정제. 200 µg/mL 농도의 액상 형태로 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CLU, 4731 |
| Immunogen | Recombinant fragment (around aa150–300) of human CLU protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Isoform 1 functions as an extracellular chaperone that prevents aggregation of nonnative proteins and inhibits amyloid fibril formation by several aggregation-prone proteins. It maintains partially unfolded proteins for refolding by other chaperones and protects cells from apoptosis and complement-mediated cytolysis. Intracellular isoforms interact with ubiquitin and E3 ligase complexes, promoting proteasomal degradation of target proteins and modulating NF-κB transcriptional activity. Nuclear isoforms promote apoptosis, while mitochondrial isoforms suppress BAX-dependent cytochrome c release and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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