Thermo Fisher Scientific Phospho-STAT3 (Tyr705) Monoclonal Antibody (LUVNKLA), FITC, eBioscience
인산화된 STAT3 (Tyr705)을 인식하는 FITC 결합 단일클론 항체로, 인간 및 마우스 시료의 세포내 염색과 유세포분석에 적합합니다. JAK/STAT 신호전달 연구에 유용하며, 488 nm 레이저에 최적화되어 있습니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Phospho-STAT3 (Tyr705) Monoclonal Antibody (LUVNKLA), FITC, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular staining)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 6 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LUVNKLA |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572521 |
Available Formats:
APC, eFluor 450, PE, PE-Cyanine7, PerCP-eFluor 710
Request custom conjugation
Product Specific Information
This LUVNKLA monoclonal antibody recognizes human and mouse STAT3 when phosphorylated on Tyr705.
STAT family proteins (STAT1, 2, 3, 4, 5A, 5B, 6) regulate apoptosis, differentiation, and proliferation in a cytokine-specific manner.
STAT3 is activated downstream of cytokines such as IFNs, IL-5, IL-6, IL-10, and LIF, and plays a key role in Th17 differentiation, cell growth, and survival.
Loss-of-function mutations reduce IL-6/IL-2-mediated proliferation, while hyperactivation is associated with poor cancer prognosis.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested for intracellular staining of stimulated human peripheral blood cells.
Use 5 µL (0.125 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).
Recommended Protocol:
- Use Protocol C: Two-step Fixation/Methanol.
- Protocol A (cytoplasmic) and Protocol B (nuclear) are not applicable.
Detailed methods available in BestProtocols®: Staining Intracellular Antigens for Flow Cytometry.
Optical Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
STAT3 is a member of the STAT protein family activated by receptor-associated kinases in response to cytokines and growth factors.
Phosphorylation at Tyr705 by JAK1/JAK2 is essential for dimerization, nuclear translocation, and DNA binding.
STAT3 mediates transcription of genes involved in cell growth and apoptosis and interacts with cofactors such as CREB-binding protein and c-Jun.
The STAT3 gene is located on chromosome 17q, and knockout models show embryonic lethality, highlighting its essential role in development.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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