Thermo Fisher Scientific S-peptide Epitope Tag Monoclonal Antibody (6.2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

10 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

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Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6.2

Immunogen

Synthetic Peptide: K(1) E T A A A K F E R Q H M D S(15) C

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_347008

Product Specific Information

This antibody detects the S-peptide in human samples.

MA1-981 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot this antibody detects the S-peptide in K562 cells. In immunohistochemistry, and immunofluorescence procedures MA1-981 recognizes the S-peptide in transfected K562 cells.

The MA1-981 immunogen corresponds to the S-peptide conjugated through a C-terminal cysteine to keyhole limpet hemocyanin.

Target Information

The S-Peptide epitope tag (S-Tag (TM)) can be used for numerous applications which include the analysis of localization, expression detection, and purification of polypeptides from mammalian cells. The small size of the tag (15 aa), the ability to purify tagged polypeptides without antibodies, and the very low expression of endogenous tissues in mammalian cells that react with S-Peptide make the S-Peptide antibody an attractive alternative to many existing epitope tags. Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.