
Thermo Fisher Scientific S-peptide Epitope Tag Monoclonal Antibody (6.2)
S-peptide 에피토프 태그를 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 합성 펩타이드 면역원 기반으로 제작되었으며, 고특이성과 재현성이 우수합니다. 단백질 발현 검출 및 정제 연구용으로 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 7 publications |
| Immunohistochemistry (IHC) | 10 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6.2 |
| Immunogen | Synthetic Peptide: K(1) E T A A A K F E R Q H M D S(15) C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_347008 |
Product Specific Information
This antibody detects the S-peptide in human samples.
MA1-981 has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures.
By Western blot, this antibody detects the S-peptide in K562 cells. In immunohistochemistry and immunofluorescence, MA1-981 recognizes the S-peptide in transfected K562 cells.
The MA1-981 immunogen corresponds to the S-peptide conjugated through a C-terminal cysteine to keyhole limpet hemocyanin.
Target Information
The S-Peptide epitope tag (S-Tag™) is used for applications such as localization analysis, expression detection, and purification of polypeptides from mammalian cells.
Its small size (15 amino acids), purification capability without antibodies, and low background in mammalian tissues make it a valuable alternative to other epitope tags.
Epitope tagging enables detection and purification of proteins expressed by cloned genes. Expression vectors are engineered with a nucleotide sequence encoding the peptide tag, producing fusion proteins that can be detected or purified using specific antibodies to the tag, removing the need for antibodies to each new protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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