Thermo Fisher Scientific Phospho-HSP27 (Ser78) Polyclonal Antibody
Phospho-HSP27 (Ser78) 특이적 항체로, 세포 내 스트레스 반응 연구에 적합. WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 인간 및 비인간 영장류에 반응하며, 고순도 항원 친화 크로마토그래피로 정제됨. 고함량 세포 기반 분석에 최적화된 제품.
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Thermo Fisher Scientific Phospho-HSP27 (Ser78) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer78 of human HSP27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 25 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11002308 |
Product Specific Information
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit.
This replacement antibody is recommended for researchers needing an antibody for high-content cell-based assays.
Validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent DyLight-conjugated secondary antibody, may be required for high-content assays.
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other phosphorylated heat shock proteins.
Target Information
Cells respond to environmental stress by increasing expression of heat shock or stress proteins.
HSPB1 (heat shock protein beta-1 or HSP27) functions as a molecular chaperone that maintains denatured proteins in a folding-competent state.
It plays roles in stress resistance and actin organization, regulating biological processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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