Thermo Fisher Scientific Phospho-DNA-PK (Thr2609) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:300,000

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 2604-2614 of human DNA PKcs corresponding to phosphorylated at the pT2609 residue if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNA-PK,uniProtId:P78527-1,ncbiNodeId:9606,antigenRange:2604-2614,antigenLength:4128,antigenImageFileName:PA1-29541_DNA-PK_P78527-1_Rabbit.svg,antigenImageFileNamePDP:PA1-29541_DNA-PK_P78527-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.93 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1957268

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.