Thermo Fisher Scientific F(ab`)2-Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:2,000-1:10,000

Immunohistochemistry (IHC)

1:1,000-1:5,000

ELISA (ELISA)

1:20,000-1:100,000

Product Specifications

Species Reactivity

Goat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Goat IgG whole molecule

Conjugate

Biotin Biotin Biotin

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Rabbit/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Rabbit/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Rabbit/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Rabbit/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Rabbit/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Rabbit/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Rabbit/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Rabbit/)
• [AP](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Rabbit/)
• [Biotin-XX](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Biotin-XX/host/Rabbit/)
• [DyLight 488](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Rabbit/)
• [DyLight 550](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Rabbit/)
• [DyLight 594](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Rabbit/)
• [DyLight 650](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Rabbit/)
• [DyLight 680](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Rabbit/)
• [DyLight 800](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977981

Target

IgG

Cross Adsorption

Against human serum proteins

Antibody Form

F(ab`)2 Fragment

Product Specific Information

PA1-29560 detects Goat IgG, heavy and light chains. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.