Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), APC, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), APC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.25 µg/test
• Publications: View 43 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 650
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form.
MAb11 is a neutralizing antibody suitable for intracellular flow cytometric analysis. Tested by intracellular staining and flow cytometry of stimulated human peripheral blood cells. Recommended use ≤ 0.25 µg/test (100 µL final volume). Optimal titration is advised.

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

TNF alpha is a multifunctional proinflammatory cytokine of the TNF superfamily, primarily secreted by macrophages. It binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors and regulates immune cell activity, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
TNF alpha exists as multimers (dimers, trimers, pentamers) and shows a single 17 kDa band under non-reducing SDS-PAGE conditions. It contributes to tumor necrosis, immune responses to infections, and has been implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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