Thermo Fisher Scientific CXCL13 Monoclonal Antibody (DS8CX13), APC, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS8CX13

Immunogen

Full length CXCL13 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL13,uniProtId:O55038-1,ncbiNodeId:10090,antigenRange:1-109,antigenLength:109,antigenImageFileName:17-7981-80_CXCL13_O55038-1_Rat.svg,antigenImageFileNamePDP:17-7981-80_CXCL13_O55038-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762701

Product Specific Information

Description: This DS8CX13 monoclonal antibody recognizes mouse CXCL13 also known as BCA-1 and Angie.

Applications Reported: This DS8CX13 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS8CX13 antibody has been tested by flow cytometric analysis of resident peritoneal macrophages. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Target Information

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyers patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitts Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.