Thermo Fisher Scientific HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience
인간 및 생쥐 HIF-1α 단백질을 검출하는 APC 결합 단일클론 항체. Flow cytometry, IF, WB 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 4°C 암저장. Hypoxia 관련 연구용으로 최적화된 항체.
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Thermo Fisher Scientific HIF-1 alpha Monoclonal Antibody (Mgc3), APC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Mgc3 |
| Immunogen | Human HIF-1 alpha amino acids 530–826 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802231 |
Product Specific Information
This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1α) from human, non-human primate, bovine, mouse, and porcine cells.
It does not cross-react with ARNT or HIF-2α.
- Validated Applications: Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, flow cytometry, gel shift assay
- Detects a ~93 kDa HIF-1α protein after hypoxic induction in COS cells
- Shows nuclear staining in COS-7 cells exposed to 1% oxygen for 4 hours
- Detected only mouse HIF-1α in gel shift assay
- Recommended replacement for Thermo Scientific Cellomics High Content Screening Kit antibody
- Optimized for cellular immunofluorescence (IF) applications
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of DFOA-stimulated HeLa cells using methanol fixation protocol
Recommended ≤ 1.0 µg/mL per test (100 µL final volume)
Cell number: 10⁵–10⁸ cells/test (empirical optimization required)
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor regulating cellular response to hypoxia.
HIF1 is composed of α and β subunits, both members of the PAS family. Under hypoxic conditions, HIF1 activates transcription of genes that enhance oxygen delivery or metabolic adaptation, such as erythropoietin, glucose transporters, VEGF, and glycolytic enzymes.
HIF1-alpha regulates:
- Hypoxia-mediated apoptosis
- Cell proliferation
- Tumor angiogenesis
It interacts with p53 and p300/CBP complexes, influencing hypoxia-responsive gene expression.
Located on chromosome 14q in humans, HIF1-alpha contributes to diseases including ischemia, cancer, pulmonary hypertension, and COPD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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