Thermo Fisher Scientific Phospho-GRF-1 (Tyr1105) Polyclonal Antibody
GRF-1 단백질의 Tyr1105 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며, Human, Mouse, Rat 시료에 반응. 1 mg/mL 농도의 액상 제품으로, PBS buffer와 glycerol 포함. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ARHGAP35 (Accession Q9NRY4), corresponding to amino acid residues around phosphorylated Tyr1105. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816507 |
Product Specific Information
Antibody detects endogenous levels of GRF-1 only when phosphorylated at Tyrosine 1105.
Target Information
RNA polymerase (Pol) III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. Rapidly growing cells require high levels of Pol III transcribed RNA, which depends on TFIIIB and TFIIIC2 transcription factor complexes.
TFIIIC2 complex (TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, TFIIIC63) is overexpressed in malignant cells and recruits RNA Pol III and TFIIIB to promoter elements, potentially contributing to deregulation in cancer.
TFIIIB complex includes TBP, TFIIIB90 (BRF1), and other factors, which are upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting TFIIIB regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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