Thermo Fisher Scientific Red Firefly Luciferase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:4,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:500-1:1,000

-

Immunoprecipitation (IP)

5 µg

-

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Species Reactivity

Luciferase

Published species

Luciferase

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified full length recombinant Red Firefly luciferase expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539910

Product Specific Information

Western blot analysis of PA1-179 detects a prominent ~62 kDa protein in Red Firefly luciferase expressing cells. No signal was detected in Green Renilla transfected or untransfected control HEK293 cells. This antibody has also been successfully tested in IP, Immunofluorescence and neutralization assays.

Red Firefly luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash
or Glow Assay Kits or Luciferase Dual Assay Kits.

Target Information

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.