Thermo Fisher Scientific Red Firefly Luciferase Polyclonal Antibody
Red Firefly luciferase 단백질을 특이적으로 인식하는 Rabbit polyclonal antibody. Western blot, ICC/IF, IP, Neutralization 등 다양한 응용 가능. 고순도 Protein G 정제 및 안정적 액상 포맷으로 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Red Firefly Luciferase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| Immunoprecipitation (IP) | 5 µg |
| Neutralization (Neu) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Luciferase |
| Published Species | Luciferase |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified full-length recombinant Red Firefly luciferase expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539910 |
Product Specific Information
Western blot analysis of PA1-179 detects a prominent ~62 kDa protein in Red Firefly luciferase expressing cells.
No signal was detected in Green Renilla transfected or untransfected control HEK293 cells.
This antibody has also been successfully tested in IP, Immunofluorescence, and Neutralization assays.
Red Firefly luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash, Glow Assay Kits, or Luciferase Dual Assay Kits.
Target Information
Luciferase produces green light with a wavelength of 562 nm.
Luciferase catalyzes the reaction:
D-firefly luciferin + O₂ + ATP → firefly oxyluciferin + CO₂ + AMP + diphosphate + light
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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